Protocol: Ampicillin Stock Solution:
Ampicillin overview:
Ampicillin is a semi-synthetic derivative of penicillin, active as a broad-spectrum antibiotic. It is inactivated by beta-lactamases and for this reason a beta-lactamase inhibitor should be considered when using ampicillin. Against gram-positive bacteria, ampicillin has a similar mode of action as benzylpenicillin; against gram-negative bacteria, it has a similar mode of action as chloramphenicol and tetracyclines. In E. coli it inhibits cell wall synthesis.
Also described for the use of inhancing luminol chemiluminescence.
Also described for the use of inhancing luminol chemiluminescence.
100mg/ml Ampicillin Stock Solution:
2. Add 10mL of sterile H2O. Dissolve completely.
3. Prewet a 0.22μm syringe filter by drawing through 5-10mL of sterile H2O and discard water.
4. Sterilize Ampicillin Stock through the prepared 0.22μm syringe filter.
5. Stock may be kept at -20C for 1 year.
